EIF4A2 Polyklonaler Antikörper

EIF4A2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 11280-1-AP

Synonyme

BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11280-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 11280-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 11280-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11280-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11280-1-AP

  IP result of anti-EIF4A2 (IP:11280-1-AP, 4ug; Detection:11280-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human breast cancer using 11280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11280-1-AP (EIF4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11280-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11280-1-AP (EIF4A2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11280-1-AP (EIF4A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Maus-Eierstockgewebe, Raji-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11280-1-AP bindet in WB, IP, IF, IHC, ELISA EIF4A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF4A2 fusion protein Ag1810
• Vollständiger Name: eukaryotic translation initiation factor 4A, isoform 2
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC015842
• Gene symbol: EIF4A2
• Gene ID (NCBI): 1974
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

Publikationen

Species	Application	Title
human	IHC, WB	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
rat	WB	Mol Ther Nucleic Acids; Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.; Authors - Zhijie Shen
mouse	WB	Stem Cell Rev Rep; Hyaluronan-CD44 Interaction Regulates Mouse Retinal Progenitor Cells Migration, Proliferation and Neuronal Differentiation; Authors - Jian Ma
human	WB	Mol Carcinog; miR-181a/b Induces the Growth, Invasion and Metastasis of Neuroblastoma Cells Through Targeting ABI1.; Authors - Xiaodan Liu
mouse	WB,IF	Biol Reprod; Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.; Authors - Jianjun Hu
human	WB	Microbes Infect; eIF4A2 is a host factor required for efficient HIV-1 replication.; Authors - Jerry Kwame Ndzinu