EIF3H Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EIF3H Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11310-1-AP

Synonyme

eIF 3 gamma, eIF3 gamma, eIF3 p40, eIF3 p40 subunit, EIF3H, EIF3S3

---

Galerie der Validierungsdaten

• 

  WB analysis using 11310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11310-1-AP (EIF3H antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, NIH/3T3-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11310-1-AP bindet in WB, IP, IF, ELISA EIF3H und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF3H fusion protein Ag1850
• Vollständiger Name: eukaryotic translation initiation factor 3, subunit H
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC000386
• Gene symbol: EIF3H
• Gene ID (NCBI): 8667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic translation initiation factor eIF3, that plays a central role in translation initiation, consists of five core subunits that are present in both the budding yeast and higher eukaryotes. The EIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex. The complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3H is a component of the EIF3 complex. [PMID:19204574,20503360]

Publikationen

Species	Application	Title
human	WB	Stem Cell Res Ther; Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.; Authors - Mengqi Chu ; KD Validated
human	WB	Biosci Biotechnol Biochem; The proliferation of colorectal cancer cells is suppressed by silencing of EIF3H.; Authors - Genhua Yu ; KD Validated
human	WB	Exp Ther Med; Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis.; Authors - Song Hong ; KD Validated
human	WB	Nat Commun; METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions; Authors - Yuanpei Li
human	WB,IF,IP	Cell Commun Signal; The deubiquitinase EIF3H promotes hepatocellular carcinoma progression by stabilizing OGT and inhibiting ferroptosis; Authors - Jianing Tang ; KD Validated