EIF3E Polyklonaler Antikörper

EIF3E Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11352-1-AP

Synonyme

Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11352-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11352-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11352-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11352-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11352-1-AP

  IP result of anti-EIF3E (IP:11352-1-AP, 4ug; Detection:11352-1-AP 1:500) with HepG2 cells lysate 880 ug.

• 

  IHC staining of human breast cancer using 11352-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11352-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11352-1-AP

  Immunofluorescent analysis of HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HepG2 using 11352-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11352-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA EIF3E und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF3E fusion protein Ag1856
• Vollständiger Name: eukaryotic translation initiation factor 3, subunit E
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-52 kDa
• GenBank-Zugangsnummer: BC000734
• Gene symbol: EIF3E
• Gene ID (NCBI): 3646
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.