EIF3B Polyklonaler Antikörper

EIF3B Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10319-1-AP

Synonyme

eIF 3 eta, EIF3 ETA, EIF3 P110, EIF3 P116, EIF3B, EIF3S9, hPrt1, PRT1, Prt1 homolog



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA375-Zellen
Erfolgreiche IPA375-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 5 publications below
IFSee 1 publications below

Produktinformation

10319-1-AP bindet in WB, IP, IF, ELISA EIF3B und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EIF3B fusion protein Ag0386
Vollständiger Name eukaryotic translation initiation factor 3, subunit B
Berechnetes Molekulargewicht 93 kDa
Beobachtetes Molekulargewicht 115 kDa
GenBank-ZugangsnummerBC001173
Gene symbol EIF3B
Gene ID (NCBI) 8662
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF3B, also named as Eukaryotic translation initiation factor 3 subunit B, is a 814 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and 8 WD repeats and belongs to the eIF-3 subunit B family. EIF3B as a RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. The calculated molecular weight of EIF3B is 93 kDa, but the phosphorylated EIF3B protein is about 115 kDa.

Protokolle

Produktspezifische Protokolle
WB protocol for EIF3B antibody 10319-1-APProtokoll herunterladen
IP protocol for EIF3B antibody 10319-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.

Authors - Yuzhang Du
humanWB

Elife

Translation initiation by the hepatitis C virus IRES requires eIF1A and ribosomal complex remodeling.

Authors - Zane A Jaafar
humanWB

Cancers (Basel)

GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.

Authors - Chiara Bellio
mouseWB

bioRxiv

Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics.

Authors - Li Wang
humanWB

Nat Commun

METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions

Authors - Yuanpei Li

Cell Chem Biol

Non-canonical function of histone methyltransferase G9a in the translational regulation of chronic inflammation

Authors - Adil Muneer