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EIF2A/CDA02 Polyklonaler Antikörper
EIF2A/CDA02 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 11233-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | MCF-7-Zellen, HEK-293T-Zellen, HeLa-Zellen, PC-12-Zellen |
Erfolgreiche IP | MCF-7-Zellen |
Erfolgreiche Detektion in IHC | humanes Prostatakarzinomgewebe, humanes Pankreasgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen, mit Ethacrynsäure behandelte HepG2-Zellen |
Erfolgreiche Detektion in FC (Intra) | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:20-1:200 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 9 publications below |
WB | See 58 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
Produktinformation
11233-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA EIF2A/CDA02 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | EIF2A/CDA02 fusion protein Ag1753 |
Vollständiger Name | eukaryotic translation initiation factor 2A, 65kDa |
Berechnetes Molekulargewicht | 65 kDa |
Beobachtetes Molekulargewicht | 65 kDa |
GenBank-Zugangsnummer | BC011885 |
Gene symbol | EIF2A |
Gene ID (NCBI) | 83939 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EIF2A, recognizes specifically the 65kd EIF2A protein. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for EIF2A/CDA02 antibody 11233-1-AP | Protokoll herunterladen |
IHC protocol for EIF2A/CDA02 antibody 11233-1-AP | Protokoll herunterladen |
IF protocol for EIF2A/CDA02 antibody 11233-1-AP | Protokoll herunterladen |
IP protocol for EIF2A/CDA02 antibody 11233-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
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Nature Translation from unconventional 5' start sites drives tumour initiation.
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Science Translation from the 5' untranslated region shapes the integrated stress response.
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Science Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.
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Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Gianluca (Verified Customer) (11-25-2021) | Good scientific feedback
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FH Daniel (Verified Customer) (04-01-2019) | The provided antibody exceeded all expectations. It worked very well on the first try.Mouse liver was homogenized in Final sample buffer, and then proteins were separated by 10% SDS-PAGE followed by western blot with 11233-1-AP(EIF2A antibody) at a dilution of 1:1000 incubated at 4 degrees Celsius over night in 5% BSA TBST.
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FH Sahya (Verified Customer) (08-13-2018) | Showed a very strong signal, will use at a higher dilution next time.
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