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  • KD/KO Validated

EIF2A/CDA02 Polyklonaler Antikörper

EIF2A/CDA02 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11233-1-AP

Synonyme

EIF2A, EIF2, EIF 2A, EIF, CDA02



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF-7-Zellen, HEK-293T-Zellen, HeLa-Zellen, PC-12-Zellen
Erfolgreiche IPMCF-7-Zellen
Erfolgreiche Detektion in IHChumanes Prostatakarzinomgewebe, humanes Pankreasgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, mit Ethacrynsäure behandelte HepG2-Zellen
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11233-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA EIF2A/CDA02 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EIF2A/CDA02 fusion protein Ag1753
Vollständiger Name eukaryotic translation initiation factor 2A, 65kDa
Berechnetes Molekulargewicht 65 kDa
Beobachtetes Molekulargewicht 65 kDa
GenBank-ZugangsnummerBC011885
Gene symbol EIF2A
Gene ID (NCBI) 83939
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EIF2A, recognizes specifically the 65kd EIF2A protein. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.

Protokolle

Produktspezifische Protokolle
WB protocol for EIF2A/CDA02 antibody 11233-1-APProtokoll herunterladen
IHC protocol for EIF2A/CDA02 antibody 11233-1-APProtokoll herunterladen
IF protocol for EIF2A/CDA02 antibody 11233-1-APProtokoll herunterladen
IP protocol for EIF2A/CDA02 antibody 11233-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB

Cell

Circadian clocks are modulated by compartmentalized oscillating translation

Authors - Yanrong Zhuang
mouseWB

Nature

Translation from unconventional 5' start sites drives tumour initiation.

Authors - Ataman Sendoel
  • KO Validated
WB

Science

Translation from the 5' untranslated region shapes the integrated stress response.

Authors - Shelley R Starck
  • KD Validated
humanWB

Science

Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.

Authors - Shelley R Starck
  • KD Validated
humanWB

Nat Cell Biol

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Authors - Yulin Chen
humanWB

Sci Adv

CReP mediates selective translation initiation at the endoplasmic reticulum.

Authors - Jonathan P Kastan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Gianluca (Verified Customer) (11-25-2021)

Good scientific feedback

  • Applications: Western Blot
FH

Daniel (Verified Customer) (04-01-2019)

The provided antibody exceeded all expectations. It worked very well on the first try.Mouse liver was homogenized in Final sample buffer, and then proteins were separated by 10% SDS-PAGE followed by western blot with 11233-1-AP(EIF2A antibody) at a dilution of 1:1000 incubated at 4 degrees Celsius over night in 5% BSA TBST.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Liver
EIF2A/CDA02 Antibody Western Blot, validation (1:1000 dilution) in Mouse Liver (Cat no:11233-1-AP)
FH

Sahya (Verified Customer) (08-13-2018)

Showed a very strong signal, will use at a higher dilution next time.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Skeletal Muscle Satellite Cells