EIF1AY Polyklonaler Antikörper

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• KD/KO Validated

EIF1AY Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11193-1-AP

Synonyme

Eukaryotic translation initiation factor 1A, Y-chromosomal, eIF-4C, eIF-1A Y isoform, eIF1A Y isoform, eIF 4C

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 11193-1-AP

  WB result of EIF1AY antibody (11193-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF1AY transfected MCF-7 cells.

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  WB analysis of MCF-7 using 11193-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11193-1-AP (EIF1AY antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11193-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11193-1-AP (EIF1AY antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 11193-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11193-1-AP (EIF1AY antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HEK-293-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11193-1-AP bindet in WB, IF/ICC, ELISA EIF1AY und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF1AY fusion protein Ag1680
• Vollständiger Name: eukaryotic translation initiation factor 1A, Y-linked
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16-20 kDa
• GenBank-Zugangsnummer: BC005248
• Gene symbol: EIF1AY
• Gene ID (NCBI): 9086
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF1AY is 1 of 5 NRY genes that are expressed in many tissues and have homologs on the X chromosome, which is an essential translation initiation factor. EIF1AY seems to be required for maximal rate of protein biosynthesis, and enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. This is a rabbit polyclonal antibody raised against the full length of human EIF1AY, and can recognize both EIF1AY and EIF1AX.