PHD1 Polyklonaler Antikörper

PHD1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 12984-1-AP

Synonyme

EGLN2, Estrogen-induced tag 6, EIT-6, EIT6, EC:1.14.11.29

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 12984-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12984-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12984-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12984-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 12984-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12984-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 12984-1-AP

  Immunofluorescent analysis of PC-3 cells using 12984-1-AP (PHD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HEK-293-Zellen, HeLa-Zellen, humanes Milzgewebe, Mausherzgewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 2 publications below

Produktinformation

12984-1-AP bindet in WB, IF/ICC, ELISA PHD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHD1 fusion protein Ag3616
• Vollständiger Name: egl nine homolog 2 (C. elegans)
• Berechnetes Molekulargewicht: 407 aa, 44 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC036051
• Gene symbol: PHD1
• Gene ID (NCBI): 112398
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGLN2, also named as PHD1, EIT6 and HPH-3, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN2 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
human	WB	Respir Res; Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin.; Authors - Changhui Yu
rat	IF	J Cell Mol Med; LncRNA MIAT enhances cerebral ischaemia/reperfusion injury in rat model via interacting with EGLN2 and reduces its ubiquitin-mediated degradation.; Authors - Suping Li
human	WB	Asian J Androl; CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancer; Authors - Yu-Zhao Wang
human	IF	Leuk Lymphoma; Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues.; Authors - Rintaro Ohe
human	WB	Cell Death Dis; Cancer associated fibroblast-derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1α/ZEB1 axis.; Authors - Haixu Xu