EFEMP2 Polyklonaler Antikörper

EFEMP2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12004-1-AP

Synonyme

Fibulin-4, Fibulin 4, FIBL-4, FIBL 4, FBLN4

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Galerie der Validierungsdaten

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  WB analysis using 12004-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12004-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12004-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12004-1-AP

  IP result of anti-EFEMP2 (IP:12004-1-AP, 4ug; Detection:12004-1-AP 1:1000) with mouse kidney tissue lysate 2160 ug.

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  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, humanes Herzgewebe, JAR-Zellen, K-562-Zellen, Mausnierengewebe, SW480-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hautgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12004-1-AP bindet in WB, IHC, IP, ELISA EFEMP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EFEMP2 fusion protein Ag2640
• Vollständiger Name: EGF-containing fibulin-like extracellular matrix protein 2
• Berechnetes Molekulargewicht: 443 aa, 49 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC010456
• Gene symbol: EFEMP2
• Gene ID (NCBI): 30008
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EFEMP2, also known as Fibulin-4 is a 60 kDa extracellular matrix (ECM) glycoprotein that is a pivotal oncogene in tumor progression and metastasis. EFEMP2 is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome.

Publikationen

Species	Application	Title
human	WB	Cancer Biol Ther; STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2; Authors - Dong Zhang
human	WB	Cell Mol Biol Lett; EFEMP2 upregulates PD-L1 expression via EGFR/ERK1/2/c-Jun signaling to promote the invasion of ovarian cancer cells; Authors - Xin Shen