CRACR2A Polyklonaler Antikörper

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• KD/KO Validated

CRACR2A Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15206-1-AP

Synonyme

CRAC channel regulator 2A, CRACR2A, EFCAB4B, Rab46

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 15206-1-AP

  WB result of CRACR2A antibody (15206-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRACR2A transfected Jurkat cells.

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  WB analysis of Jurkat using 15206-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15206-1-AP (CRACR2A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15206-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15206-1-AP (CRACR2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 15206-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 15206-1-AP (CRACR2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15206-1-AP

  IP Result of anti-CRACR2A (IP:15206-1-AP, 4ug; Detection:15206-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of human tonsillitis using 15206-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15206-1-AP (CRACR2A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15206-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15206-1-AP (CRACR2A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 15206-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 15206-1-AP (CRACR2A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Daudi-Zellen, HeLa-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

15206-1-AP bindet in WB, IP, IHC, IF, ELISA CRACR2A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRACR2A fusion protein Ag7358
• Vollständiger Name: EF-hand calcium binding domain 4B
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 80-90 kDa
• GenBank-Zugangsnummer: BC004524
• Gene symbol: EFCAB4B
• Gene ID (NCBI): 84766
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.

Publikationen

Species	Application	Title
human	WB,IP	Elife; Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder.; Authors - Beibei Wu
mouse	WB	J Immunol; CRACR2A-Mediated TCR Signaling Promotes Local Effector Th1 and Th17 Responses.; Authors - Jin Seok Woo
human	IP,IF,WB	Sci Rep; Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells.; Authors - Lucia Pedicini
human	WB	Biochem Biophys Res Commun; A long variant of CRACR2A that belongs to the Rab GTPase protein family.; Authors - Lesley A Wilson ; KD Validated
human	WB,IF	J Cell Biol; Rab46 integrates Ca2+ and histamine signaling to regulate selective cargo release from Weibel-Palade bodies.; Authors - Katarina T Miteva ; KD Validated

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