eEF2K Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

eEF2K Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13510-1-AP

Synonyme

eEF-2K, eEF-2 kinase, eEF 2K, eEF 2 kinase, EC:2.7.11.20

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 13510-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13510-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13510-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 13510-1-AP

  IP result of anti-eEF2K (IP:13510-1-AP, 4ug; Detection:13510-1-AP 1:500) with PC-3 cells lysate 3200ug.

• 

  IF Staining of PC-3 using 13510-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13510-1-AP (EEF2K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13510-1-AP bindet in WB, IF/ICC, IP, CoIP, ELISA eEF2K und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: eEF2K fusion protein Ag4432
• Vollständiger Name: eukaryotic elongation factor-2 kinase
• Berechnetes Molekulargewicht: 725 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC032665
• Gene symbol: EEF2K
• Gene ID (NCBI): 29904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cancer Lett; eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.; Authors - Cheng Ji
human	WB	Oncol Lett; AMPK decreases ERK1/2 activity and cancer cell sensitivity to nutrition deprivation by mediating a positive feedback loop involving eEF2K.; Authors - Shujuan Tong
rat	WB,IP,CoIP	Cells; SIRT1 Protects the Heart from ER Stress-Induced Injury by Promoting eEF2K/eEF2-Dependent Autophagy.; Authors - Julie Pires Da Silva ; KD Validated
human	WB	Med Oncol; A drug repurposing study identifies novel FOXM1 inhibitors with in vitro activity against breast cancer cells; Authors - Khaled A N Abusharkh
		J Mol Med (Berl); FOXM1 plays a role in autophagy by transcriptionally regulating Beclin-1 and LC3 genes in human triple-negative breast cancer cells.; Authors - Zuhal Hamurcu