EEF1D Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EEF1D Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 10630-1-AP

Synonyme

Antigen NY CO 4, EEF1D, EF 1 delta, EF 1D, EF1D, Elongation factor 1 delta, FP1047

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Galerie der Validierungsdaten

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  WB analysis using 10630-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10630-1-AP (EEF1D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10630-1-AP

  IP result of anti-EEF1D (IP:10630-1-AP, 3ug; Detection:10630-1-AP 1:300) with HeLa cells lysate 1200ug.

• 

  IP experiment of HeLa using 10630-1-AP

  IP result of anti-EEF1D (IP:10630-1-AP, 3ug; Detection:10630-1-AP 1:300) with HeLa cells lysate 1200ug.

• 

  IHC staining of human pancreas cancer using 10630-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10630-1-AP (EEF1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10630-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10630-1-AP (EEF1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10630-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10630-1-AP (EEF1D antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, U2OS-Zellen, U-87 MG-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• CoIP: See 1 publications below

Produktinformation

10630-1-AP bindet in WB, IP, IHC, CoIP, ELISA EEF1D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EEF1D fusion protein Ag0983
• Vollständiger Name: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC007847
• Gene symbol: EEF1D
• Gene ID (NCBI): 1936
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

Publikationen

Species	Application	Title
human	WB	Cell Chem Biol; A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.; Authors - Yingjie Hou
human,mouse	WB	EMBO Rep; Transformation of eEF1Bδ into heat-shock response transcription factor by alternative splicing.; Authors - Kaitsuka Taku T ; KD Validated
human	WB	J Exp Clin Cancer Res; EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling.; Authors - Dong-Dong Cheng ; KD Validated
mouse	WB,CoIP	Front Mol Biosci; Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1.; Authors - Taku Kaitsuka
mouse	WB	Int J Biochem Cell Biol; The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis.; Authors - Zhu Zijue Z
rat	WB	Proteomics; Pulmonary pressure reduction attenuates expression of proteins identified by lung proteomic profiling in pulmonary hypertensive rats.; Authors - ?stergaard Louise L

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (03-12-2020)

Sharp and strong band for western blot

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart