EEF1B2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EEF1B2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10095-2-AP

Synonyme

Elongation factor 1 beta, EF-1-beta, EF1B, EF 1 beta, EEF1B1

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Galerie der Validierungsdaten

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  WB analysis using 10095-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10095-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10095-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10095-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10095-2-AP

  WB result of EEF1B2 antibody (10095-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells.

• 

  WB analysis of RAW264.7 using 10095-2-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10095-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 10095-2-AP

  IP result of anti-EEF1B2 (IP:10095-2-AP, 4ug; Detection:10095-2-AP 1:6000) with Jurkat cells lysate 1400 ug.

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  IHC staining of human pancreas cancer using 10095-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10095-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10095-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10095-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10095-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10095-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10095-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EEF1B2 antibody (10095-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

10095-2-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA EEF1B2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EEF1B2 fusion protein Ag0135
• Vollständiger Name: eukaryotic translation elongation factor 1 beta 2
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC000211
• Gene symbol: EEF1B2
• Gene ID (NCBI): 1933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human EEF1B2.

Publikationen

Species	Application	Title
	RIP	Nat Cell Biol; The MBNL3 splicing factor promotes hepatocellular carcinoma by increasing PXN expression through the alternative splicing of lncRNA-PXN-AS1.; Authors - Ji-Hang Yuan
human	WB,IF,IP	Mol Cell Biol; Translational repression by RNA-binding protein TIAR.; Authors - Mazan-Mamczarz Krystyna K
human	WB	Front Microbiol; Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus Proteins.; Authors - Shuhei Sammaibashi
mouse, human	WB, IHC, IF	PLoS One; Characterisation of Translation Elongation Factor eEF1B Subunit Expression in Mammalian Cells and Tissues and Co-Localisation with eEF1A2.; Authors - Yuan Cao ; KD Validated
human	WB	Biotechniques; En masse analysis of nascent translation using microarrays.; Authors - Mazan-Mamczarz Krystyna K