EED Monoklonaler Antikörper

EED Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D11D4

Kat-Nr. : 67756-1-Ig

Synonyme

EED, embryonic ectoderm development, HEED, Polycomb protein EED, WAIT 1, WAIT1

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Galerie der Validierungsdaten

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  WB analysis using 67756-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67756-1-Ig (EED antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of mouse testis using 67756-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67756-1-Ig (EED antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 67756-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67756-1-Ig (EED antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 67756-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EED antibody (67756-1-Ig, Clone: 1D11D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse testis using 67756-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EED antibody (67756-1-Ig, Clone: 1D11D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, HEK--293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67756-1-Ig bindet in WB, IHC, IF, ELISA EED und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EED fusion protein Ag10369
• Vollständiger Name: embryonic ectoderm development
• Berechnetes Molekulargewicht: 466 aa, 53 kDa
• Beobachtetes Molekulargewicht: 50/53/46 kDa.
• GenBank-Zugangsnummer: BC068995
• Gene symbol: EED
• Gene ID (NCBI): 8726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.