DPPA2 Polyklonaler Antikörper

DPPA2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12689-1-AP

Synonyme

ECSA, Pluripotent embryonic stem cell-related gene 1 protein, PESCRG1, Developmental pluripotency-associated protein 2

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 12689-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12689-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12689-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12689-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 12689-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 12689-1-AP (DPPA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

12689-1-AP bindet in WB, IF/ICC, ELISA DPPA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DPPA2 fusion protein Ag3601
• Vollständiger Name: developmental pluripotency associated 2
• Berechnetes Molekulargewicht: 298 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC018070
• Gene symbol: DPPA2
• Gene ID (NCBI): 151871
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	IF	Mol Hum Reprod; Differential expression of the embryo/cancer gene ECSA(DPPA2), the cancer/testis gene BORIS and the pluripotency structural gene OCT4, in human preimplantation development.; Authors - Monk Marilyn M