RCAS1 Polyklonaler Antikörper

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• KD/KO Validated

RCAS1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12255-1-AP

Synonyme

EBAG9, PDAF, Estrogen receptor-binding fragment-associated gene 9 protein, EBAG 9, EB9

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Galerie der Validierungsdaten

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  WB analysis using 12255-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12255-1-AP (RCAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12255-1-AP

  WB result of RCAS1 antibody (12255-1-AP, 1:10000) with si-control and si-RCAS1 transfected HEK293 cells.

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  IP experiment of HeLa using 12255-1-AP

  IP result of anti-RCAS1 (IP:12255-1-AP, 4ug; Detection:12255-1-AP 1:2000) with HeLa cells lysate 1720 ug.

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  IHC staining of human colon using 12255-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 12255-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 12255-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using RCAS1 antibody (12255-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

12255-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RCAS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RCAS1 fusion protein Ag2905
• Vollständiger Name: estrogen receptor binding site associated, antigen, 9
• Berechnetes Molekulargewicht: 213 aa, 24 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC017729
• Gene symbol: RCAS1
• Gene ID (NCBI): 9166
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Estrogen receptor-binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.

Publikationen

Species	Application	Title
rat	WB,IHC	J Ethnopharmacol; Exploring the inhibitory impact of Mongolian medicinal He-Zi-3 soup on mammary gland hyperplasia in rats induced by estrogen and progestogen; Authors - Chunlan Liang