EAF1 Polyklonaler Antikörper

EAF1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13787-1-AP

Synonyme

ELL-associated factor 1

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 13787-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13787-1-AP (EAF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13787-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13787-1-AP (EAF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13787-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13787-1-AP (EAF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13787-1-AP

  IP result of anti-EAF1 (IP:13787-1-AP, 4ug; Detection:13787-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of SH-SY5Y using 13787-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13787-1-AP (EAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, Maushirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IP: See 1 publications below

Produktinformation

13787-1-AP bindet in WB, IF/ICC, IP, ELISA EAF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EAF1 fusion protein Ag4804
• Vollständiger Name: ELL associated factor 1
• Berechnetes Molekulargewicht: 268 aa, 29 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC041329
• Gene symbol: EAF1
• Gene ID (NCBI): 85403
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. The tumor suppressor EAF2 is regulated by androgen signaling and associated with prostate cancer. Eaf factors have been revealed to modulate mesoderm and neural patterning by inhibiting canonical Wnt signaling, and high activity of canonical Wnt/β-catenin is revealed in eafs morphants (PMID: 23364330). Catalog#13787-1-AP is a rabbit polyclonal antibody raised against the full-length of human EAF1.

Publikationen

Species	Application	Title
human	WB	Mol Cell; HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP.; Authors - Bijan Sobhian
human	WB	Cell Death Differ; Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.; Authors - Ping Liu
	WB	Biochim Biophys Acta; Transcriptional factors Eaf1/2 inhibit endoderm and mesoderm formation via suppressing TGF-β signaling.; Authors - Jing-Xia Liu
human	WB	Biochim Biophys Acta Gene Regul Mech; ELL-associated factors EAF1/2 negatively regulate HIV-1 transcription through inhibition of Super Elongation Complex formation.; Authors - Rongdiao Liu
mouse	WB,IP	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao