DYRK1B Polyklonaler Antikörper

DYRK1B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12240-1-AP

Synonyme

DYRK1B, Minibrain related kinase, MIRK, Mirk protein kinase

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 12240-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12240-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 12240-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12240-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12240-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HeLa-Zellen, humanes Herzgewebe, humanes Kolongewebe, Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12240-1-AP bindet in WB, ELISA DYRK1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DYRK1B fusion protein Ag2883
• Vollständiger Name: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B
• Berechnetes Molekulargewicht: 629 aa, 69 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC018751
• Gene symbol: DYRK1B
• Gene ID (NCBI): 9149
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DYRK1B(dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B) is also named as MIRK. It is a member of the dyrk/minibrain family of serine/threonine kinases that mediate the transition from growth to differentiation in lower eukaryotes and mammals(PMID:15546868). DYRK1B functions as a transcriptional coactivator that is activated by co-expressed MKK3, a MAP kinase that also can activate p38 MAP kinase(PMID:14500717). The DYRK1B splice variants found in each muscle tissue exhibit approximate molecular weights of 70 and 75 kDa(PMID:12902328). It has 3 isoforms produced by alternative splicing of the molecular weight of 65-69 kDa.

Publikationen

Species	Application	Title
human	WB	Am J Physiol Cell Physiol; MicroRNA-9 enhances the transactivation of nuclear factor of activated T cells by targeting KPNB1 and DYRK1B.; Authors - Yan Zeng