DVL3 Polyklonaler Antikörper

DVL3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13444-1-AP

Synonyme

Dishevelled 3, Segment polarity protein dishevelled homolog DVL-3, KIAA0208, DSH homolog 3, Dishevelled-3

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Galerie der Validierungsdaten

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  WB analysis using 13444-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13444-1-AP (DVL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13444-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13444-1-AP (DVL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 13444-1-AP

  IP result of anti-DVL3 (IP:13444-1-AP, 4ug; Detection:13444-1-AP 1:500) with Raji cells lysate 1440ug.

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  IHC staining of human colon cancer using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13444-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DVL3 antibody (13444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13444-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13444-1-AP (DVL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Prostatakarzinomgewebe, humanes Zervixkarzinomgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

13444-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DVL3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Krallenfrosch (Xenopus)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DVL3 fusion protein Ag4272
• Vollständiger Name: dishevelled, dsh homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 716 aa, 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC032459
• Gene symbol: DVL3
• Gene ID (NCBI): 1857
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
Xenopus	WB,IP	J Cell Biol; Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled.; Authors - Moonsup Lee
mouse	IF	J Cell Biol; Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling.; Authors - Andre Landin Malt
human	WB	Oncogene; The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.; Authors - Kai Li
human	WB	Cancer Lett; SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network.; Authors - Li Xuefei X
human	WB	Oncotarget; Dalbinol, a rotenoid from Amorpha fruticosa L., exerts anti-proliferative activity by facilitating β-catenin degradation in hepatocellular carcinoma cells.; Authors - Xiaohui Zhu
human	WB,IHC,IF	Biol Proced Online; Circadian gene CSNK1D promoted the progression of hepatocellular carcinoma by activating Wnt/β-catenin pathway via stabilizing Dishevelled Segment Polarity Protein 3; Authors - Mengqi Zhu