DUT Polyklonaler Antikörper

DUT Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13740-1-AP

Synonyme

EC:3.6.1.23, dUTPase, deoxyuridine triphosphatase, Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

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Galerie der Validierungsdaten

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  WB analysis using 13740-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13740-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 13740-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13740-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13740-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:700 incubated at 4 degree celsius over night.

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  IHC staining of human heart using 13740-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13740-1-AP (DUT Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 13740-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13740-1-AP (DUT Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 13740-1-AP

  Immunofluorescent analysis of HEK-293 cells using 13740-1-AP (DUT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Lebergewebe, humanes Nierengewebe, Ramos-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

13740-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA DUT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DUT fusion protein Ag4677
• Vollständiger Name: deoxyuridine triphosphatase
• Berechnetes Molekulargewicht: 252 aa, 27 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC033645
• Gene symbol: DUT
• Gene ID (NCBI): 1854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,CoIP	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
human	WB	Cell Chem Biol; CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity.; Authors - Ying Yu Liang
human	WB	Bioconjug Chem; ATP-Linked Chimeric Nucleotide as a Specific Luminescence Reporter of Deoxyuridine Triphosphatase.; Authors - Debin Ji