• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DUSP16 Polyklonaler Antikörper

DUSP16 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14237-1-AP

Synonyme

DUSP16, KIAA1700, MAP kinase phosphatase 7, MKP 7, MKP7

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of PC-12 cells using DUSP16 Polyclonal antibody (14237-1-AP)

    WB analysis of PC-12 using 14237-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HeLa cells using DUSP16 Polyclonal antibody (14237-1-AP)

    WB analysis of HeLa using 14237-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of COLO 320 cells using DUSP16 Polyclonal antibody (14237-1-AP)

    WB analysis of COLO 320 using 14237-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse brain tissue using DUSP16 Polyclonal antibody (14237-1-AP)

    WB analysis of mouse brain using 14237-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human colon tissue using DUSP16 Polyclonal antibody (14237-1-AP)

    IHC staining of human colon using 14237-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using DUSP16 Polyclonal antibody (14237-1-AP)

    IHC staining of human colon using 14237-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DUSP16 Polyclonal antibody (14237-1-AP)

    IHC staining of human breast cancer using 14237-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DUSP16 Polyclonal antibody (14237-1-AP)

    IHC staining of human breast cancer using 14237-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:50 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBPC-12-Zellen, COLO 320-Zellen, HeLa-Zellen, Maushirngewebe
Erfolgreiche Detektion in IHChumanes Kolongewebe, humanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

14237-1-AP bindet in WB, IHC,ELISA DUSP16 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte ReaktivitätMaus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DUSP16 fusion protein Ag5479
Vollständiger Name dual specificity phosphatase 16
Berechnetes Molekulargewicht 73 kDa
Beobachtetes Molekulargewicht 70 kDa
GenBank-ZugangsnummerBC042101
Gene symbol DUSP16
Gene ID (NCBI) 80824
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DUSP16, also known as MKP-7, is a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. DUSP16 dephosphorylates JUN kinases (JNKs) specifically and thereby regulates T-cell differentiation, which can be responsible for the pathogenesis of Burkitt's lymphoma and cerebral ischemia. DUSP16 was also found to induce the expression of VCAM-1 through the suppression of JNK signaling.

Protokolle

Produktspezifische Protokolle
WB protocol for DUSP16 antibody 14237-1-APProtokoll herunterladen
IHC protocol for DUSP16 antibody 14237-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Cell Death Discov

NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving.

Authors - Ze-Qing Pu
View Article