Dysbindin Polyklonaler Antikörper

Dysbindin Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11132-1-AP

Synonyme

DBND, DTNBP1, DTNBP1a, DTNBP1b, Dysbindin, Dysbindin 1, dystrobrevin binding protein 1, HPS7, HPS7 protein, My031, SDY

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 11132-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11132-1-AP (Dysbindin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11132-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11132-1-AP (Dysbindin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11132-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11132-1-AP (Dysbindin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11132-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11132-1-AP (Dysbindin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11132-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11132-1-AP (Dysbindin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hodengewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

11132-1-AP bindet in WB, IF, IHC, ELISA Dysbindin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Dysbindin fusion protein Ag1613
• Vollständiger Name: dystrobrevin binding protein 1
• Berechnetes Molekulargewicht: 351 aa, 39 kDa
• Beobachtetes Molekulargewicht: 48 kDa, 36 kDa
• GenBank-Zugangsnummer: BC011912
• Gene symbol: Dysbindin
• Gene ID (NCBI): 84062
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dysbindin, encoded by DTNBP1 (dystrobrevin binding protein 1), is a synaptic protein which is expressed in diverse neuronal populations throughout the brain. Genetic variation in DTNBP1 is associated with schizophrenia. Reduced expression of dysbindin has been reported in the dorsolateral prefrontal cortex (DLPFC) of schizophrenia cases. Several isoforms of dysbindin exist: 48-50 kDa of dysbindin-1A, 35-38 kDa of dysbindin-1B, and 30-32 kDa of dysbindin-1C. This antibody recognizes all of isoforms of dysbindin. (19617633)

Publikationen

Species	Application	Title
		J Cell Biol; BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia.; Authors - William J Monis
mouse	WB	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook
mouse	WB	Neuropharmacology; Mice with dopaminergic neuron-specific deletion of DTNBP-1 gene show blunted nucleus accumbens dopamine release and associated behaviors.; Authors - Sanjeev K Bhardwaj
human	WB	Neuroscience; Protein kinase B/Akt1 phosphorylates dysbindin-1A at serine 10 to regulate neuronal development.; Authors - Erkang Fei
human	WB	Br J Pharmacol; Dopaminergic signaling and behavioral alterations by Comt-Dtnbp1 genetic interaction and their clinical relevance; Authors - Francesca Managò
mouse	WB,IF	Transl Psychiatry; Deletion of Dtnbp1 in mice impairs threat memory consolidation and is associated with enhanced inhibitory drive in the amygdala.; Authors - Cathy C Y Huang