DSG2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DSG2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 21880-1-AP

Synonyme

ARVC10, ARVD10, Cadherin family member 5, CDHF5, desmoglein 2, DSG2, HDGC

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 21880-1-AP

  WB result of DSG2 antibody (21880-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DSG2 transfected HEK-293 cells.

• 

  WB analysis of HepG2 using 21880-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21880-1-AP (DSG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 21880-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21880-1-AP (DSG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21880-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21880-1-AP (DSG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21880-1-AP

  WB result of DSG2 antibody (21880-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DSG2 transfected HeLa cells.

• 

  WB analysis of HEK-293 using 21880-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21880-1-AP (DSG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 21880-1-AP

  IP Result of anti-DSG2 (IP:21880-1-AP, 4ug; Detection:21880-1-AP 1:1000) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human lung cancer using 21880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21880-1-AP (DSG2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21880-1-AP (DSG2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 21880-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DSG2 antibody (21880-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 21880-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 21880-1-AP (DSG2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A431-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 4 publications below

Produktinformation

21880-1-AP bindet in WB, IP, IHC, IF, ELISA DSG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DSG2 fusion protein Ag16393
• Vollständiger Name: desmoglein 2
• Berechnetes Molekulargewicht: 1118 aa, 122 kDa
• Beobachtetes Molekulargewicht: 145-150 kDa
• GenBank-Zugangsnummer: BC099655
• Gene symbol: DSG2
• Gene ID (NCBI): 1829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).

Publikationen

Species	Application	Title
human	IF	EMBO Rep; O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.; Authors - Ieva Bagdonaite
human	WB	Virol Sin; Desmoglein 2 (DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia.; Authors - Jing Zhang ; KD Validated
human	IF	Helicobacter; Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact.; Authors - Chen Zhang
human	IF	Mol Biol Cell; Plakophilin 3 and Par3 Facilitate Desmosomes' association with the Apical Junctional Complex.; Authors - Indrajyoti Indra
mouse	WB	Food Chem Toxicol; Sodium fluoride and sulfur dioxide affected male reproduction by disturbing blood-testis barrier in mice.; Authors - Jianhai Zhang
mouse	WB	Microscopy (Oxf); Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.; Authors - Shoko Ishikawa

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Saba (Verified Customer) (09-26-2022)

it is said that this antibody is rabbit, however, unfortunately we observed a significant cross reaction with secondary anti mouse antibody.

• Applications: Immunofluorescence

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