DR4 Polyklonaler Antikörper

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• KD/KO Validated

DR4 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 24063-1-AP

Synonyme

APO2, CD261, Death receptor 4, DR4, TNFRSF10A, TRAIL R1, TRAIL receptor 1, TRAILR 1, TRAILR1

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Galerie der Validierungsdaten

• 

  WB analysis using 24063-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24063-1-AP (DR4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 24063-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24063-1-AP (DR4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24063-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24063-1-AP (DR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 24063-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24063-1-AP (DR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 12 publications below

Produktinformation

24063-1-AP bindet in WB, ELISA DR4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DR4 fusion protein Ag20927
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 10a
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 37-55 kDa
• GenBank-Zugangsnummer: BC012866
• Gene symbol: TNFRSF10A
• Gene ID (NCBI): 8797
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily. TRAIL activates apoptosis through the death receptors DR4 (also known as TRAILR1 and TNFRSF10A) and DR5 (also known as TRAILR2, KILLER and TNFRSF10B). DR4 and DR5 are single-pass type I membrane proteins that contain intracellular death domains (DD) and upon activation mediate apoptotic signals (PMID: 11163110). Binding of TRAIL to DR4 or DR5 results in receptor oligomerization and recruitment of FAS-associated protein with death domain (FADD) and caspase 8 to form a functional death-inducing signalling complex (DISC). Upon DISC formation, caspase 8 is cleaved and activated, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis (PMID: 18813321). DR4 or DR5 promotes the activation of NF-kappa-B and play an important role in inflammation (PMID: 9430227, 19434100).

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; Celastrol induces apoptosis and autophagy via the ROS/JNK signaling pathway in human osteosarcoma cells: an in vitro and in vivo study.; Authors - H-Y Li
human	WB	Am J Cancer Res; PMID: 31598394; Authors - Ran An
human	WB	Biomed Pharmacother; Pro-survival effects by NF-κB, Akt and ERK(1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer.; Authors - Juan Yang
human	WB	Oncogenesis; JWA regulates TRAIL-induced apoptosis via MARCH8-mediated DR4 ubiquitination in cisplatin-resistant gastric cancer cells.; Authors - Q Wang
human	WB	Mar Drugs; Fucoidan Induces Apoptosis of HT-29 Cells via the Activation of DR4 and Mitochondrial Pathway.; Authors - Xu Bai
human	WB	J Ethnopharmacol; Combination of Compound Kushen Injection and cisplatin shows synergistic antitumor activity in p53-R273H/P309S mutant colorectal cancer cells through inducing apoptosis.; Authors - Xingkang Wu

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