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DPP9 Polyklonaler Antikörper

DPP9 Polyklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11568-1-AP

Synonyme

dipeptidyl peptidase 9, Dipeptidyl peptidase IX, DP9, DPLP9, DPP IX, DPP9, DPRP 2, DPRP2

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HepG2 cells using DPP9 Polyclonal antibody (11568-1-AP)

    WB analysis of HepG2 using 11568-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DPP9 Polyclonal antibody (11568-1-AP)

    WB analysis of MCF-7 using 11568-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using DPP9 Polyclonal antibody (11568-1-AP)

    WB analysis of mouse heart using 11568-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using DPP9 Polyclonal antibody (11568-1-AP)

    WB analysis of A2780 using 11568-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using DPP9 Polyclonal antibody (11568-1-AP)

    WB analysis of PC-3 using 11568-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DPP9 Polyclonal antibody (11568-1-AP)

    IHC staining of human ovary tumor using 11568-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11568-1-AP (DPP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DPP9 Polyclonal antibody (11568-1-AP)

    IHC staining of human ovary tumor using 11568-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11568-1-AP (DPP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DPP9 Polyclonal antibody (11568-1-AP)

    IF Staining of HepG2 using 11568-1-AP

    Immunofluorescent analysis of HepG2 cells using 11568-1-AP (DPP9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using DPP9 Polyclonal antibody (11568-1-AP)

    IF Staining of PC-3 using 11568-1-AP

    Immunofluorescent analysis of PC-3 cells using 11568-1-AP (DPP9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DPP9 Polyclonal antibody (11568-1-AP)

    IF Staining of MCF-7 using 11568-1-AP

    Immunofluorescent analysis of MCF-7 cells using 11568-1-AP (DPP9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, A2780-Zellen, Mausherzgewebe, MCF-7-Zellen, PC-3-Zellen
Erfolgreiche Detektion in IHChumanes Ovarialkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, MCF-7-Zellen, PC-3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11568-1-AP bindet in WB, IF, IHC, ELISA DPP9 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DPP9 fusion protein Ag2145
Vollständiger Name dipeptidyl-peptidase 9
Berechnetes Molekulargewicht 863 aa, 98 kDa
Beobachtetes Molekulargewicht 98 kDa
GenBank-ZugangsnummerBC037948
Gene symbol DPP9
Gene ID (NCBI) 91039
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dipeptidyl peptidase 9(DPP9), is a member of the S9B family in clan SC of the serine proteases. DPP9 has 3 isoforms with MW of 95, 98 and 101 kDa. DPP9 is involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer.