DNAJC2/MPP11 Polyklonaler Antikörper

DNAJC2/MPP11 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11971-1-AP

Synonyme

DNAJC2, DnaJ homolog subfamily C member 2, N-terminally processed, ZRF1, MPP11, MPHOSPH11

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Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 11971-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11971-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11971-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11971-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11971-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11971-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11971-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11971-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11971-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11971-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11971-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11971-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11971-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11971-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11971-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11971-1-AP

  IP result of anti-DNAJC2/MPP11 (IP:11971-1-AP, 4ug; Detection:11971-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human liver cancer using 11971-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11971-1-AP (DNAJC2/MPP11 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11971-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11971-1-AP (DNAJC2/MPP11 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11971-1-AP

  Immunofluorescent analysis of HepG2 cells, using DNAJC2 antibody 11971-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Mauslebergewebe, PC-3-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

11971-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DNAJC2/MPP11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJC2/MPP11 fusion protein Ag2594
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily C, member 2
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 66-75 kDa
• GenBank-Zugangsnummer: BC056682
• Gene symbol: DNAJC2
• Gene ID (NCBI): 27000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	EMBO J; Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins.; Authors - Guiyou Tian
human	WB	Cancers (Basel); Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin; Authors - Mohamed Alalem