DNAJC19 Polyklonaler Antikörper

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• KD/KO Validated

DNAJC19 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 12096-1-AP

Synonyme

TIMM14, TIM14

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12096-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12096-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12096-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12096-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12096-1-AP

  IP result of anti-DNAJC19 (IP:12096-1-AP, 4ug; Detection:12096-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

• 

  IF Staining of HepG2 using 12096-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DNAJC19 antibody (12096-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, humanes Lungengewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• FC: See 1 publications below

Produktinformation

12096-1-AP bindet in WB, IF/ICC, IP, ELISA DNAJC19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJC19 fusion protein Ag2739
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily C, member 19
• Berechnetes Molekulargewicht: 116 aa, 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC009702
• Gene symbol: DNAJC19
• Gene ID (NCBI): 131118
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJC19 is a mitochondrial cochaperone that interacts with HSP70 chaperones through its conserved J-domain. As a transmembrane protein, DNAJC19 is strongly associated with the inner mitochondrial membrane. Mutations in DNAJC19 cause dilated cardiomyopathy with ataxia (DCMA). Recently DNAJC19 has been reported as interactor of PHB complex to regulate cardiolipin remodeling, which addressed the link of DNAJC19 to cardiomyopathy. This antibody is specific to DNAJC19 and had been tested by siRNA.(24856930)

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	Cell Metab; DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.; Authors - Ricarda Richter-Dennerlein ; KD Validated
human	WB	Hum Mol Genet; Defining functional classes of Barth syndrome mutation in humans.; Authors - Ya-Wen Lu
human		Stem Cell Res; Generation of two patient-derived iPSC lines from siblings (LIBUCi001-A and LIBUCi002-A) and a genetically modified iPSC line (JMUi001-A-1) to mimic dilated cardiomyopathy with ataxia (DCMA) caused by a homozygous DNAJC19 mutation.; Authors - Anna Janz
human	WB	Cell Rep; The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.; Authors - Michelle Grace Acoba
human	WB	Cell Rep; LPGAT1 controls MEGDEL syndrome by coupling phosphatidylglycerol remodeling with mitochondrial transport; Authors - Haoran Sun