DNAJB6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DNAJB6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 11707-1-AP

Synonyme

HSJ2, HSJ 2, HHDJ1, Heat shock protein J2, DnaJ

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Galerie der Validierungsdaten

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  WB analysis using 11707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11707-1-AP (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11707-1-AP

  WB result of DNAJB6 antibody (11707-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJB6 transfected HeLa cells.

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  WB analysis using 11707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11707-1-AP (DNAJB6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11707-1-AP

  IP result of anti-DNAJB6 (IP:11707-1-AP, 4ug; Detection:11707-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 11707-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11707-1-AP (DNAJB6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11707-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11707-1-AP (DNAJB6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11707-1-AP

  Immunofluorescent analysis of HepG2 cells, using DNAJB6 antibody 11707-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, Maushodengewebe, Rattenhirngewebe, SH-SY5Y-Zellen, Y79-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

11707-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DNAJB6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJB6 fusion protein Ag2258
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 6
• Berechnetes Molekulargewicht: 241 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa, 38 kDa
• GenBank-Zugangsnummer: BC000177
• Gene symbol: DNAJB6
• Gene ID (NCBI): 10049
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID:20159555).

Publikationen

Species	Application	Title
human	WB	Neurobiol Dis; DNAJB6 suppresses alpha-synuclein induced pathology in an animal model of Parkinson's disease.; Authors - Sertan Arkan
human,mouse	WB,IHC,IF	J Parkinsons Dis; DNAJB6b is Downregulated in Synucleinopathies.; Authors - Jonas Folke ; KO Validated
mouse	WB,IF	iScience; Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.; Authors - Qianxing Zou
human	WB,IHC	Mol Carcinog; Overexpression of DNAJB6 promotes colorectal cancer cell invasion through an IQGAP1/ERK-dependent signaling pathway.; Authors - Tong-Tong Zhang ; KD Validated
mouse	WB,IF	STAR Protoc; Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse.; Authors - Qianxing Zou
human	WB	Cancers (Basel); Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin; Authors - Mohamed Alalem