DNAJA2 Polyklonaler Antikörper

DNAJA2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 12236-1-AP

Synonyme

HIRA interacting protein 4, Dnj3, DNAJ, DJA2, Dj3

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Galerie der Validierungsdaten

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  WB analysis of A375 using 12236-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12236-1-AP (DNAJA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12236-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12236-1-AP (DNAJA2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12236-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12236-1-AP (DNAJA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 12236-1-AP

  IP result of anti-DNAJA2 (IP:12236-1-AP, 4ug; Detection:12236-1-AP 1:300) with A375 cells lysate 3600 ug.

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  IF Staining of MCF-7 using 12236-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 12236-1-AP (DNAJA2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, humanes Hirngewebe, Mausnierengewebe
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

12236-1-AP bindet in WB, IF/ICC, IP, CoIP, ELISA DNAJA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJA2 fusion protein Ag2877
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily A, member 2
• Berechnetes Molekulargewicht: 417 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC015809
• Gene symbol: DNAJA2
• Gene ID (NCBI): 10294
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Metamorphism in TDP-43 prion-like domain determines chaperone recognition; Authors - Jaime Carrasco
human	WB	Int J Cancer; Down-regulation of BiP/GRP78 sensitizes resistant prostate cancer cells to gene-therapeutic overexpression of REIC/Dkk-3.; Authors - Tanimoto Ryuta R
human	WB	J Biol Chem; A cytosolic chaperone complex controls folding and degradation of type III CD38.; Authors - Yang Wu
human	WB	Cancers (Basel); Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin; Authors - Mohamed Alalem
human,mouse	WB,IP	J Biol Chem; Quantitative interactome proteomics identifies a proteostasis network for GABAA receptors; Authors - Ya-Juan Wang
mouse	WB	Cancer Res; N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma; Authors - Duygu Kuzuoglu-Ozturk