DMC1 Polyklonaler Antikörper

DMC1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 13714-1-AP

Synonyme

LIM15, HsLim15, DMC1H

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Galerie der Validierungsdaten

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  WB analysis using 13714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13714-1-AP (DMC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13714-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13714-1-AP (DMC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13714-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13714-1-AP (DMC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 13714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13714-1-AP (DMC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13714-1-AP (DMC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13714-1-AP

  Immunofluorescent analysis of HeLa cells using 13714-1-AP (DMC1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HeLa-Zellen, humanes Hodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 9 publications below

Produktinformation

13714-1-AP bindet in WB, IHC, IF/ICC, ELISA DMC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DMC1 fusion protein Ag4653
• Vollständiger Name: DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
• Berechnetes Molekulargewicht: 165 aa, 18 kDa, 38 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC035658
• Gene symbol: DMC1
• Gene ID (NCBI): 11144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Nucleic Acids Res; NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.; Authors - Lu Chen
mouse	IF	Nucleic Acids Res; MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis.; Authors - Yongliang Shang
		Cell Prolif; ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression; Authors - Xu Zhou
mouse	IF	Cell Death Differ; NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.; Authors - Bin Zhang
mouse	IF	Elife; A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1.; Authors - Natalia Felipe-Medina
human	IHC	Front Oncol; The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL).; Authors - Jennifer Gantchev