DLAT Polyklonaler Antikörper

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• KD/KO Validated

DLAT Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

38

Kat-Nr. : 13426-1-AP

Synonyme

PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

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Galerie der Validierungsdaten

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  WB analysis using 13426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13426-1-AP

  WB result of DLAT antibody (13426-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLAT transfected HeLa cells.

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  WB analysis using 13426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13426-1-AP

  IP result of anti-DLAT (IP:13426-1-AP, 3ug; Detection:13426-1-AP 1:1000) with HepG2 cells lysate 2000ug.

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  IHC staining of human liver cancer using 13426-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13426-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13426-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13426-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13426-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (13426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 27 publications below
• IHC: See 12 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13426-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA DLAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DLAT fusion protein Ag4226
• Vollständiger Name: dihydrolipoamide S-acetyltransferase
• Berechnetes Molekulargewicht: 647 aa, 70 kDa
• Beobachtetes Molekulargewicht: 69-70 kDa
• GenBank-Zugangsnummer: BC039084
• Gene symbol: DLAT
• Gene ID (NCBI): 1737
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
mouse	WB	Nat Cell Biol; RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis; Authors -
human,mouse	WB,IF,CoIP	Mol Cell; Acetyl-CoA production by Mediator-bound 2-ketoacid dehydrogenases boosts de novo histone acetylation and is regulated by nitric oxide; Authors - Marta Russo
human	IHC	Cell Biosci; A new thinking: deciphering the aberrance and clinical implication of copper-death signatures in clear cell renal cell carcinoma; Authors - Aimin Jiang
human	WB,IHC	Cancer Cell Int; Molecular subtypes based on cuproptosis-related genes and tumor microenvironment infiltration characteristics in pancreatic adenocarcinoma; Authors - Jian Li
		J Biol Chem; Decreased pyruvate dehydrogenase activity in Tafazzin-deficient cells is caused by dysregulation of pyruvate dehydrogenase phosphatase 1 (PDP1); Authors - Zhuqing Liang