DIRAS1 Polyklonaler Antikörper

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• KD/KO Validated

DIRAS1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12634-1-AP

Synonyme

Di Ras1, DIRAS1, GBTS1, GTP binding protein Di Ras1, RIG

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Galerie der Validierungsdaten

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  WB analysis of human brain using 12634-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12634-1-AP (DIRAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12634-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12634-1-AP (DIRAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12634-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12634-1-AP (DIRAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12634-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12634-1-AP (DIRAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12634-1-AP

  IP result of anti-DIRAS1 (IP:12634-1-AP, 4ug; Detection:12634-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human oesophagus using 12634-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 12634-1-AP (DIRAS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus using 12634-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 12634-1-AP (DIRAS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, humanes Plazenta-Gewebe, MCF-7-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Ösophagusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Produktinformation

12634-1-AP bindet in WB, IP, IHC, ELISA DIRAS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DIRAS1 fusion protein Ag3329
• Vollständiger Name: DIRAS family, GTP-binding RAS-like 1
• Berechnetes Molekulargewicht: 198 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22-24 kDa
• GenBank-Zugangsnummer: BC030660
• Gene symbol: DIRAS1
• Gene ID (NCBI): 148252
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DIRAS1 belongs to the small guanosine triphosphatase (GTPase) Ras superfamily. DIRAS1 is frequently downregulated in human cancers due to DNA copy number loss, loss of heterozygosity (LOH), and hypermethylation of the promoter. The restoration of DIRAS1 has been reported to suppress tumor growth and metastasis in human neural tumors, esophageal cancer, colorectal cancer, and ovarian cancer.

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas.; Authors - Tanja Rothhammer-Hampl
human	WB,IHC	Mol Ther Nucleic Acids; RNAa and Vector-Mediated Overexpression of DIRAS1 Suppresses Tumor Growth and Migration in Renal Cell Carcinoma.; Authors - Xin Xu ; KD Validated
human	WB	J Biol Chem; The Tumor Suppressive Small GTPase DiRas1 Binds the Non-canonical Guanine Nucleotide Exchange Factor SmgGDS and Antagonizes SmgGDS Interactions with Oncogenic Small GTPases.; Authors - Carmen Bergom
rat	WB,IHC	Int J Environ Res Public Health; Effects of In Utero Exposure to Di-n-Butyl Phthalate on Testicular Development in Rat.; Authors - Tan Ma
human	WB,IHC	Cancer Biol Ther; M6A modification regulates tumor suppressor DIRAS1 expression in cervical cancer cells; Authors - Yu-Yan Wang