mDia1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

mDia1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Rezensionen (1)

Kat-Nr. : 20624-1-AP

Synonyme

DFNA1, DIA1, DIAP1, DIAPH1, Diaphanous related formin 1, DRF1, hDIA1, LFHL1, mDia1, Protein diaphanous homolog 1

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Galerie der Validierungsdaten

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  WB analysis using 20624-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 20624-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 20624-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 20624-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20624-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20624-1-AP

  IP Result of anti-mDia1 (IP:20624-1-AP, 4ug; Detection:20624-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of mouse kidney using 20624-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20624-1-AP (mDia1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20624-1-AP

  Immunofluorescent analysis of HepG2 cells, using DIAPH1 antibody 20624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 20624-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using mDia1 antibody (20624-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20624-1-AP

  Immunofluorescent analysis of Hela cells, using DIAPH1 antibody 20624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 20624-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human mDia1 (20624-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COS-7-Zellen, humanes Herzgewebe, humanes Skelettmuskelgewebe, HUVEC-Zellen, MDA-MB-231-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• ChIP: See 1 publications below

Produktinformation

20624-1-AP bindet in WB, IP, IHC, IF, FC, ChIP, ELISA mDia1 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: mDia1 fusion protein Ag14523
• Vollständiger Name: diaphanous homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 1272 aa, 141 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa, 70 kDa
• GenBank-Zugangsnummer: BC007411
• Gene symbol: DIAPH1
• Gene ID (NCBI): 1729
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).

Publikationen

Species	Application	Title
mouse	WB,IHC,IF	Circ Res; Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways.; Authors - Touré Fatouma F
mouse	WB	Dev Cell; Force-dependent activation of actin elongation factor mDia1 protects the cytoskeleton from mechanical damage and promotes stress fiber repair.; Authors - Fernando R Valencia ; KD Validated
human	WB,IHC	J Cell Biol; Dia1 coordinates differentiation and cell sorting in a stratified epithelium.; Authors - Robert M Harmon
human	WB, IF	Mol Biol Cell; Small molecule agonists of mammalian Diaphanous-related (mDia) formins reveal an effective glioblastoma anti-invasion strategy.; Authors - Jessica D Arden
human	WB	Cells; The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.; Authors - Kathryn N Becker
human	WB	PLoS One; A Novel Role for p115RhoGEF in Regulation of Epithelial Plasticity.; Authors - Swapnil S Kher ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (11-20-2023)

Detects a band around 150kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

mDia1 Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:20624-1-AP)