DHX29 Polyklonaler Antikörper

DHX29 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 13923-1-AP

Synonyme

DDX29, DEAH box protein 29, DHX29, Nucleic acid helicase DDXx

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 13923-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13923-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13923-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 13923-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13923-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13923-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, HT-1080.Zellen, K-562-Zellen, MCF-7-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13923-1-AP bindet in WB, ELISA DHX29 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHX29 fusion protein Ag4938
• Vollständiger Name: DEAH (Asp-Glu-Ala-His) box polypeptide 29
• Berechnetes Molekulargewicht: 155 kDa
• Beobachtetes Molekulargewicht: 150-155 kDa
• GenBank-Zugangsnummer: BC056219
• Gene symbol: DHX29
• Gene ID (NCBI): 54505
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lilas (Verified Customer) (06-29-2021)

Used antibody at a 1:1000 dilution in 5% BSA, and overnight incubation at 4 degrees. A very sharp band detected at around 155 kDA using western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma cell lines