DGKH Polyklonaler Antikörper

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• KD/KO Validated

DGKH Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13873-1-AP

Synonyme

EC:2.7.1.107, DGK-eta, DGKeta, DAG kinase eta

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 13873-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13873-1-AP (DGKH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13873-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13873-1-AP (DGKH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13873-1-AP

  IP result of anti-DGKH (IP:13873-1-AP, 5ug; Detection:13873-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human testis using 13873-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13873-1-AP (DGKH Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13873-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13873-1-AP (DGKH Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of SH-SY5Y using 13873-1-AP

  Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 13873-1-AP (DGKH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13873-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DGKH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DGKH fusion protein Ag4926
• Vollständiger Name: diacylglycerol kinase, eta
• Berechnetes Molekulargewicht: 1100 aa, 122 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC043292
• Gene symbol: DGKH
• Gene ID (NCBI): 160851
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DGKH(Diacylglycerol kinase eta) is also named as DGK-eta and belongs to the eukaryotic diacylglycerol kinase family. It is a key protein in the lithium-sensitive phosphatidyl inositol pathway, and recently a link between environmental stress and schizophrenia/bipolar disorder has been revealed. DGKH has some isoforms produced by alternative splicing. Two isoforms DGKH1 and DGKH2 with the molecular weight of 128 kDa and 135 kDa shows diacylglycerol kinase activity; however, the 2 isoforms differed in several other characteristics(PMID:12810723).

Publikationen

Species	Application	Title
human, mouse	WB	J Invest Dermatol; Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes.; Authors - Kawaguchi Masakazu M
mouse	WB	BMC Dev Biol; Distinct expression and localization of the type II diacylglycerol kinase isozymes ¿, ¿ and ¿ in the mouse reproductive organs.; Authors - Takao Shionoya
mouse	WB	J Histochem Cytochem; Expression and Localization of Type II Diacylglycerol Kinase Isozymes δ and η in the Developing Mouse Brain.; Authors - Takako Usuki
human, mouse	WB,IHC	Clin Transl Oncol; Diacylglycerol kinase η modulates oncogenic properties of lung cancer cells.; Authors - Nakano T T ; KD Validated
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Myristic acid specifically stabilizes diacylglycerol kinase δ protein in C2C12 skeletal muscle cells.; Authors - Kai Iwata
human	WB	Cancer Med; Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma; Authors - Yimin Sun