DGAT1 Polyklonaler Antikörper

DGAT1 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (3)

Kat-Nr. : 11561-1-AP

Synonyme

ACAT related gene product 1, AGRP1, ARGP1, DGAT, DGAT1, Diglyceride acyltransferase

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Galerie der Validierungsdaten

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  IHC staining of human hepatocirrhosis using 11561-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11561-1-AP (DGAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 11561-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11561-1-AP (DGAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

11561-1-AP bindet in WB, IF, IHC, ELISA DGAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DGAT1 fusion protein Ag2123
• Vollständiger Name: diacylglycerol O-acyltransferase homolog 1 (mouse)
• Berechnetes Molekulargewicht: 488 aa, 55 kDa
• Beobachtetes Molekulargewicht: 50-57 kDa
• GenBank-Zugangsnummer: BC015762
• Gene symbol: DGAT1
• Gene ID (NCBI): 8694
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Commun; Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.; Authors - Fangjun Yu
mouse	WB	J Cachexia Sarcopenia Muscle; Exogenous H2 S promotes ubiquitin-mediated degradation of SREBP1 to alleviate diabetic cardiomyopathy via SYVN1 S-sulfhydration; Authors - Shiwu Zhang
human	IHC	Front Oncol; Identification of an Aging-Related Gene Signature in Predicting Prognosis and Indicating Tumor Immune Microenvironment in Breast Cancer.; Authors - Wenchang Lv
mouse	WB,IF	J Virol; Lipid droplets are beneficial for rabies virus replication by facilitating viral budding; Authors - Jianqing Zhao
mouse,rat	WB	J Cell Mol Med; Hydrogen sulphide reduced the accumulation of lipid droplets in cardiac tissues of db/db mice via Hrd1 S-sulfhydration.; Authors - Yu Sun
mouse	IHC	Lab Invest; Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice.; Authors - Karuna Irungbam

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kelly (Verified Customer) (09-06-2024)

Great antibody

• Applications: Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: IMMUNOPRECIPITATION
• Cell Tissue Type: Antibody

FH

Kelly (Verified Customer) (09-04-2024)

• Applications: Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: IMMUNOFLUORESCENCE
• Cell Tissue Type: Antibody

FH

Kel (Verified Customer) (07-04-2023)

Great product.

• Applications: Immunofluorescence, Protein purification