DFNA5/GSDME Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DFNA5/GSDME Polyklonal Antikörper für WB, IP, IHC, ELISA, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IF, IHC, CoIP, ELISA, FC (Intra)

Konjugation

Unkonjugiert

Publikationen

34

Rezensionen (2)

Kat-Nr. : 13075-1-AP

Synonyme

Cleaved Gasdermin E, deafness, autosomal dominant 5, DFNA5, DFNA5/GSDME, Gasdermin E, Gasdermin-E, GSDME, ICERE 1, ICERE1

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Galerie der Validierungsdaten

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  WB analysis using 13075-1-AP

  Untreated, and etoposide (60uM, 5h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13075-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13075-1-AP

  WB result of DFNA5/ GSDME antibody (13075-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DFNA5/ GSDME transfected A549 cells.

• 

  WB analysis using 13075-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 13075-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 13075-1-AP

  IP result of anti-DFNA5/GSDME (IP:13075-1-AP, 4ug; Detection:13075-1-AP 1:15000) with SH-SY5Y cells lysate 1240 ug.

• 

  IHC staining of mouse brain using 13075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 13075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 13075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 13075-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DFNA5/GSDME (13075-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, A549-Zellen, HeLa-Zellen, Y79-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 14 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Produktinformation

13075-1-AP bindet in WB, IP, IF, IHC, CoIP, ELISA, FC (Intra) DFNA5/GSDME und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DFNA5/GSDME fusion protein Ag3746
• Vollständiger Name: deafness, autosomal dominant 5
• Berechnetes Molekulargewicht: 496 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa, 35 kDa, 25 kDa
• GenBank-Zugangsnummer: BC019689
• Gene symbol: DFNA5
• Gene ID (NCBI): 1687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

Publikationen

Species	Application	Title
human,mouse	WB,IHC,CoIP	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
human	IF	Acta Pharm Sin B; A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer; Authors - Yang Liu
mouse	WB	J Control Release; Enhancing cell pyroptosis with biomimetic nanoparticles for melanoma chemo-immunotherapy; Authors - Shiquan Sun
mouse	IF	Cell Death Differ; GSDME-mediated pyroptosis promotes inflammation and fibrosis in obstructive nephropathy.; Authors - Yinshuang Li
mouse	WB,IHC	J Nanobiotechnology; Hydralazine loaded nanodroplets combined with ultrasound-targeted microbubble destruction to induce pyroptosis for tumor treatment; Authors - Shuting Huang
		Cell Death Dis; TNFSF15 inhibits progression of diabetic retinopathy by blocking pyroptosis via interacting with GSDME; Authors - Hongkun Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (11-18-2022)

Used for GSDME detection in splenocyte extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Spleen

DFNA5/GSDME Antibody Western Blot validation (1/1000 dilution) in Spleen (Cat no:13075-1-AP)

FH

Evelina (Verified Customer) (03-14-2022)

Good antibody to detect GSDME in cell lysates. Although does detect one of the proteins within the ladder (approx.15kDa) when using stronger ECL.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

DFNA5/GSDME Antibody Western Blot validation (1:1000 dilution) in (Cat no:13075-1-AP)

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C