Desmin Polyklonaler Antikörper

Desmin Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22205-1-AP

Synonyme

Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 22205-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22205-1-AP (Desmin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22205-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22205-1-AP (Desmin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Skelettmuskelgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22205-1-AP bindet in WB, IHC, ELISA Desmin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Desmin fusion protein Ag17525
• Vollständiger Name: desmin
• Berechnetes Molekulargewicht: 470 aa, 54 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC032116
• Gene symbol: DES
• Gene ID (NCBI): 1674
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.