DDX50 Polyklonaler Antikörper

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• KD/KO Validated

DDX50 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10358-1-AP

Synonyme

Nucleolar protein Gu2, Gu-beta, Gu beta, EC:3.6.4.13, DEAD box protein 50

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10358-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10358-1-AP (DDX50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10358-1-AP

  WB result of DDX50 antibody (10358-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX50 transfected HeLa cells.

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  WB analysis of Jurkat using 10358-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10358-1-AP (DDX50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10358-1-AP

  IP result of anti-DDX50 (IP:10358-1-AP, 4ug; Detection:10358-1-AP 1:800) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 10358-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX50 antibody (10358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

10358-1-AP bindet in WB, IF/ICC, IP, ELISA DDX50 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX50 fusion protein Ag0382
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC000272
• Gene symbol: DDX50
• Gene ID (NCBI): 79009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DEAD box polypeptide 50 (DDX50, synonyms: GU2, GUB, MGC3199, RH-II/GuB) enzyme may be involved in ribosomal RNA synthesis or processing.

Publikationen

Species	Application	Title
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	Arch Virol; DDX50 inhibits the replication of dengue virus 2 by upregulating IFN-β production.; Authors - Peijun Han
human	WB	Mol Cell; Nucleolus activity-dependent recruitment and biomolecular condensation by pH sensing; Authors - Fardin Aryan