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DDX4,VASA Polyklonaler Antikörper

DDX4,VASA Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12888-1-AP

Synonyme

DDX4, DEAD box protein 4, VASA, Vasa homolog

Galerie der Validierungsdaten

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDX4/VASA Polyclonal antibody (12888-1-AP)

    IHC staining of mouse testis using 12888-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12888-1-AP (DDX4/VASA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDX4/VASA Polyclonal antibody (12888-1-AP)

    IHC staining of mouse testis using 12888-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12888-1-AP (DDX4/VASA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Geprüfte Anwendungen

Produktinformation

12888-1-AP bindet in IHC, ELISA DDX4,VASA und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DDX4,VASA fusion protein Ag3919
Vollständiger Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Berechnetes Molekulargewicht690aa,76 kDa; 724aa,79 kDa
GenBank-ZugangsnummerBC047455
Gene symbol DDX4
Gene ID (NCBI) 54514
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].