DDX3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DDX3 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Rezensionen (1)

Kat-Nr. : 11115-1-AP

Synonyme

DDX3X, DEAD box protein 3, X-chromosomal, DDX14, DBX, CAP-Rf

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Galerie der Validierungsdaten

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  WB analysis using 11115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11115-1-AP

  WB result of DDX3 antibody (11115-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells.

• 

  WB analysis using 11115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11115-1-AP

  WB result of DDX3 antibody (11115-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HeLa cells.

• 

  WB analysis of mouse liver using 11115-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11115-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 11115-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11115-1-AP

  IP result of anti-DDX3 (IP:11115-1-AP, 4ug; Detection:11115-1-AP 1:300) with HeLa cells lysate 2480ug.

• 

  IHC staining of human malignant melanoma using 11115-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 11115-1-AP (DDX3 antibody) at dilution of 1:200 (under 40x lens)..

• 

  IHC staining of human malignant melanoma using 11115-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 11115-1-AP (DDX3 antibody) at dilution of 1:200 (under 10x lens)..

• 

  IHC staining of human cervical cancer using 11115-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11115-1-AP (DDX3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11115-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDX3 antibody (11115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11115-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX3 antibody (11115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 11115-1-AP

  Immunofluorescent analysis of HepG2 cells using 11115-1-AP (DDX3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of HeLa using 11115-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DDX3 (11115-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, HepG2-Zellen, HT-1080.Zellen, K-562-Zellen, Maushirngewebe, Mauslebergewebe, NIH/3T3-Zellen, PC-12-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 23 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• CoIP: See 4 publications below
• RIP: See 2 publications below

Produktinformation

11115-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA DDX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX3 fusion protein Ag1614
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC011819
• Gene symbol: DDX3
• Gene ID (NCBI): 1654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

Publikationen

Species	Application	Title
human	WB,CoIP	Cell; Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules; Authors - Qinqin Cui
	IF	Neuron; Pathogenic DDX3X Mutations Impair RNA Metabolism and Neurogenesis during Fetal Cortical Development.; Authors - Ashley L Lennox
human	WB	Neuron; CRISPR-Cas9 Screens Identify the RNA Helicase DDX3X as a Repressor of C9ORF72 (GGGGCC)n Repeat-Associated Non-AUG Translation.; Authors - Weiwei Cheng ; KD Validated
human	WB	Cancer Lett; NR4A1 depletion inhibits colorectal cancer progression by promoting necroptosis via the RIG-I-like receptor pathway; Authors - Jinghan Zhu
human	WB	Pharmacol Res; HPV E7-drived ALKBH5 promotes cervical cancer progression by modulating m6A modification of PAK5; Authors - Fu-Chun Huo ; KD Validated
mouse	WB,IF,CoIP	Ecotoxicol Environ Saf; Aluminum exposure induces central nervous system impairment via activating NLRP3-medicated pyroptosis pathway; Authors - Wudi Hao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shannon (Verified Customer) (07-19-2022)

Worked well, also binds to different isoforms/protein dimers

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Jurkat T-Cells

DDX3 Antibody Western Blot,Immunoprecipitation validation (1:2000 dilution) in Jurkat T-Cells (Cat no:11115-1-AP)