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DDX20 Polyklonaler Antikörper

DDX20 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11324-1-AP

Synonyme

Gemin 3, EC:3.6.1.15, DP103, DEAD box protein DP 103, DEAD box protein 20



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushodengewebe
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, HeLa-Zellen
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:20-1:200
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11324-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA DDX20 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DDX20 fusion protein Ag1863
Vollständiger Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Berechnetes Molekulargewicht 824 aa, 92 kDa
Beobachtetes Molekulargewicht 100 kDa
GenBank-ZugangsnummerBC011556
Gene symbol DDX20
Gene ID (NCBI) 11218
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (DDX20), also known as DP103 or Gemin3, is a member of the DEAD box protein family expressed ubiquitously. DEAD family proteins use energy from ATP hydrolysis for RNA chaperoning and RNPase activity (PMID: 27121695). As a core member of the survival motor neuron (SMN) complex, DDX20 participate in small nuclear ribonucleoprotein (snRNP) biogenesis. Second, DDX20 have direct roles in gene expression in view of its implication in transcription and post-transcriptional gene silencing. Addition, the false expression of DDX20 could have deleterious effects on cellular homeostasis thus leading to cancer development and progression (PMID:29523774). Anymore, DDX20 could be identified as a biomarker and metastasis-driving oncogene of human breast cancer (PMID: 25083991). The detected weight of DDX20 is slightly higher than the theoretical molecular weight that is because of phosphorylation after translation.

Protokolle

Produktspezifische Protokolle
WB protocol for DDX20 antibody 11324-1-APProtokoll herunterladen
IHC protocol for DDX20 antibody 11324-1-APProtokoll herunterladen
IF protocol for DDX20 antibody 11324-1-APProtokoll herunterladen
IP protocol for DDX20 antibody 11324-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB, IHC

J Clin Invest

DEAD-box helicase DP103 defines metastatic potential of human breast cancers.

Authors - Eun Myoung Shin
  • KD Validated
mouseWB,IHC,IF

Dev Cell

DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice

Authors - Dingfeng Zou
humanIHC

Aging (Albany NY)

An artificial neural network model to diagnose non-obstructive azoospermia based on RNA-binding protein-related genes

Authors - Fan Peng
humanWB

Comput Struct Biotechnol J

Analysis of the expression, function, prognosis and co-expression genes of DDX20 in gastric cancer.

Authors - Qingshui Wang
humanIHC

Arthritis Res Ther

Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies.

Authors - Ping Li
WB

Biosci Rep

FOXL2 Down-regulates Vitellogenin Expression at Mature Stage in Eriocheir sinensis.

Authors - Qing Li