DOPA decarboxylase Polyklonaler Antikörper

DOPA decarboxylase Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 10166-1-AP

Synonyme

DDC, EC:4.1.1.28, Aromatic-L-amino-acid decarboxylase, AADC

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Galerie der Validierungsdaten

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  WB analysis using 10166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10166-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10166-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10166-1-AP

  IP result of anti-DOPA decarboxylase (IP:10166-1-AP, 4ug; Detection:10166-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of rat small intestine using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10166-1-AP (DOPA decarboxylase antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10166-1-AP (DOPA decarboxylase antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 10166-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DOPA decarboxylase antibody (10166-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe, Mausnierengewebe, PC-12-Zellen, Rattennierengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Leberkarzinomgewebe, Ratten-Dünndarmgewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Produktinformation

10166-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DOPA decarboxylase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DOPA decarboxylase fusion protein Ag0219
• Vollständiger Name: dopa decarboxylase (aromatic L-amino acid decarboxylase)
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC008366
• Gene symbol: DOPA decarboxylase
• Gene ID (NCBI): 1644
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aromatic-L-amino-acid decarboxylase belongs to the pyridoxal-dependent aminotransferase superfamily.DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD).Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.

Publikationen

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
human,mouse	WB,IHC	Cell Signal; Profile of 5-HT2A receptor involved in signaling cascades associated to intracellular inflammation and apoptosis in hepatocytes and its role in carbon tetrachloride-induced hepatotoxicity; Authors - Yuxin Zhang
human	WB	Dis Model Mech; A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease.; Authors - Zhao-Feng Li
mouse	IHC,IF	Toxicol Appl Pharmacol; PMID: 21703292; Authors - Fox Donald A DA
human	WB	J Transl Med; Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's disease.; Authors - Ming Ming
human,mouse	WB,IHC	Toxicol Appl Pharmacol; Carbon tetrachloride induced mitochondrial division, respiratory chain damage, abnormal intracellular [H+] and apoptosis are due to the activation of 5-HT degradation system in hepatocytes.; Authors - Yuxin Zhang