DDB2 Polyklonaler Antikörper

DDB2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30173-1-AP

Synonyme

DDB p48 subunit, DDB2, DDBB, DNA damage binding protein 2, FLJ34321, UV DDB 2, UV DDB2

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Galerie der Validierungsdaten

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  WB analysis using 30173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30173-1-AP (DDB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30173-1-AP (DDB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30173-1-AP (DDB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Mauslebergewebe, Mausnierengewebe, NIH/3T3-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30173-1-AP bindet in WB, ELISA DDB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDB2 fusion protein Ag32811
• Vollständiger Name: damage-specific DNA binding protein 2, 48kDa
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-51 kDa
• GenBank-Zugangsnummer: NM_000107
• Gene symbol: DDB2
• Gene ID (NCBI): 1643
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDB2, also named as DNA damage-binding protein 2 or UV-damaged DNA-binding protein 2, is a 427 amino acid protein, which contains seven WD repeats and belongs to the WD repeat DDB2/WDR76 family. DDB2 is ubiquitously expressed, with highest levels in corneal endothelium and lowest levels in brain. DDB2 localizes in the nucleus and is required for DNA repair. DDB2 regulates negatively the constitutive expression of the SOD2 gene in breast cancer cells and exerts, at least in part, a control of breast cancer cell growth. DDB2 can play a role as oncogene and may become a promising candidate as a predictive marker in breast cancer.