DDB1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DDB1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 11380-1-AP

Synonyme

DDB p127 subunit, DDB1, DDBA, DNA damage binding protein 1, DNA damage binding protein a, HBV X associated protein 1, UV damaged DNA binding factor, UV DDB 1, UV DDB1, XAP 1, XAP1, XPCE, XPE, XPE BF, XPE binding factor

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Galerie der Validierungsdaten

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  WB analysis using 11380-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11380-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11380-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11380-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11380-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 11380-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 11380-1-AP

  IP result of anti-DDB1 (IP:11380-1-AP, 5ug; Detection:11380-1-AP 1:500) with Jurkat cells lysate 3080ug.

• 

  IHC staining of human colon cancer using 11380-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11380-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11380-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11380-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, C2C12-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, humanes Nierengewebe, humanes Plazenta-Gewebe, Jurkat-Zellen, Maushodengewebe, MCF-7-Zellen, MDA-MB-231-Zellen, NIH/3T3-Zellen, Rattenhodengewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

11380-1-AP bindet in WB, IP, IHC, CoIP, ChIP, ELISA DDB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDB1 fusion protein Ag1901
• Vollständiger Name: damage-specific DNA binding protein 1, 127kDa
• Berechnetes Molekulargewicht: 1140 aa, 127 kDa
• Beobachtetes Molekulargewicht: 127 kDa
• GenBank-Zugangsnummer: BC011686
• Gene symbol: DDB1
• Gene ID (NCBI): 1642
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.

Publikationen

Species	Application	Title
mouse	WB	Front Immunol; Ddb1 Is Essential for the Expansion of CD4+ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.; Authors - Lingtao Yang
	CoIP	iScience; A Multidimensional Characterization of E3 Ubiquitin Ligase and Substrate Interaction Network.; Authors - Di Chen
human	WB	PLoS One; 14-3-3ε mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage.; Authors - Tang Siwei S
human	WB	Virology; DDB1 is a cellular substrate of NS3/4A protease and required for hepatitis C virus replication.; Authors - Kang Xi X ; KD Validated
human	WB	Cell Death Dis; Cul4 E3 ubiquitin ligase regulates ovarian cancer drug resistance by targeting the antiapoptotic protein BIRC3.; Authors - Xiaoyan Hu ; KD Validated
mouse		Cell Rep; SENP3 and USP7 regulate Polycomb-rixosome interactions and silencing functions; Authors - Haining Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah (Verified Customer) (07-03-2019)

Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-DDB1 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U251/Jurkat

DDB1 Antibody Western Blot, validation (1:1000 dilution) in U251/Jurkat (Cat no:11380-1-AP)