DCX Polyklonaler Antikörper

DCX Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Rezensionen (3)

Kat-Nr. : 13925-1-AP

Synonyme

Lis-X, Lissencephalin-X, Doublin, doublecortin

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Galerie der Validierungsdaten

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  WB analysis using 13925-1-AP

  Fetal human brain lysates were subjected to SDS PAGE followed by western blot with 13925-1-AP (DCX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13925-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13925-1-AP (DCX antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 13925-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 13925-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 13925-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed SH-SY5Y cells using 13925-1-AP (DCX antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, fetales humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 22 publications below

Produktinformation

13925-1-AP bindet in WB, IHC, IF/ICC, ELISA DCX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCX fusion protein Ag4945
• Vollständiger Name: doublecortin
• Berechnetes Molekulargewicht: 402 aa, 45 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC027925
• Gene symbol: DCX
• Gene ID (NCBI): 1641
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.; Authors - Ming Zhang
human	WB	PLoS Pathog; Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells; Authors - Xian-Zhang Wang
mouse	IF	Brain Behav Immun; Bacteroides species differentially modulate depression-like behavior via gut-brain metabolic signaling.; Authors - Youying Zhang
mouse	IF	J Neuroinflammation; Exosomes derived from bone-marrow mesenchymal stem cells alleviate cognitive decline in AD-like mice by improving BDNF-related neuropathology.; Authors - Sen Liu
mouse	IF	Neurotherapeutics; Fecal Microbiota Transplantation from Aged Mice Render Recipient Mice Resistant to MPTP-Induced Nigrostriatal Degeneration Via a Neurogenesis-Dependent but Inflammation-Independent Manner; Authors - Chen-Meng Qiao
mouse	IF	Mol Neurobiol; miR-377-3p Regulates Hippocampal Neurogenesis via the Zfp462-Pbx1 Pathway and Mediates Anxiety-Like Behaviors in Prenatal Hypoxic Offspring; Authors - Bin Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sampath (Verified Customer) (12-02-2019)

It worked very well brain samples and gave me a strong signal for DCX at 1:1000 dilution for western blots. I had to increase the dilution to 1:2000. This is the antibody I was looking for. If you are looking for DCX antibody, I will recommend this.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000

FH

Sheed (Verified Customer) (01-26-2019)

Works well with Edu click reaction

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse

FH

Eleonora (Verified Customer) (12-11-2018)

The antibody works well, but it has too much interaction with the protein ladder (I have used Fisher BioReagent EZ-RUN).

• Applications: Western Blot,
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: cortex (right) and hippocampus (left) mouse 3 weeks

DCX Antibody Western Blot, validation (1:600 dilution) in cortex (right) and hippocampus (left) mouse 3 weeks (Cat no:13925-1-AP)