DBT Polyklonaler Antikörper

DBT Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 12451-1-AP

Synonyme

BCKDHE2, BCKAD-E2, BCKADE2, BCATE2, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis

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Galerie der Validierungsdaten

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  WB analysis using 12451-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12451-1-AP (DBT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12451-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12451-1-AP (DBT antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 12451-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12451-1-AP (DBT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 12451-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12451-1-AP (DBT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12451-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DBT antibody (12451-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maushirngewebe, NCI-H1299-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1500-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IP: See 1 publications below

Produktinformation

12451-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DBT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DBT fusion protein Ag3101
• Vollständiger Name: dihydrolipoamide branched chain transacylase E2
• Berechnetes Molekulargewicht: 482 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC016675
• Gene symbol: DBT
• Gene ID (NCBI): 1629
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nat Metab; Branched-chain amino acid catabolism in muscle affects systemic BCAA levels but not insulin resistance; Authors - Megan C Blair
mouse	WB,IP	J Nutr; Pyruvate Kinase M2 Supports Muscle Progenitor Cell Proliferation but Is Dispensable for Skeletal Muscle Regeneration after Injury.; Authors - Jamie E Blum
human,mouse	WB	Heliyon; Hyperactivation of mTOR and AKT in a cardiac hypertrophy animal model of Friedreich ataxia; Authors - Wing-Hang Tong
mouse	WB	Cell Metab; Extra-cardiac BCAA catabolism lowers blood pressure and protects from heart failure; Authors - Danielle Murashige
human	WB	Front Immunol; Overexpression of DBT suppresses the aggressiveness of renal clear cell carcinoma and correlates with immune infiltration; Authors - Chiyu Zhang
mouse	WB	J Thromb Haemost; Branched-chain amino acids promote thrombocytopoiesis by activating mTOR signaling; Authors - Haojie Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Maria (Verified Customer) (08-15-2021)

DBT in human primary fibroblasts. 10 ug of total protein. 4-15% TGX gel. Blocking 3% BSA in PBST (0.1% Tween). 1:1000 primary ab O/N incubation at 4ºC. 1:5000 secondary ab HRP conj. 1h RT in BSA 3% in PBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary fibroblasts

DBT Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:12451-1-AP)