HIP55 Polyklonaler Antikörper

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• KD/KO Validated

HIP55 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13015-1-AP

Synonyme

DBNL, HPK1-interacting protein of 55 kDa, Drebrin-like protein, Drebrin-F, Cervical mucin-associated protein

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 13015-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13015-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13015-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13015-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13015-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13015-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13015-1-AP

  IP result of anti-HIP55 (IP:13015-1-AP, 4ug; Detection:13015-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human breast cancer using 13015-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13015-1-AP (DBNL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13015-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13015-1-AP (DBNL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13015-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HIP55 antibody (13015-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 13015-1-AP

  Immunofluorescent analysis of HepG2 cells, using DBNL antibody 13015-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green).

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  FC experiment of HepG2 using 13015-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HIP55 Polyclonal antibody (13015-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, humanes Herzgewebe, Jurkat-Zellen, K-562-Zellen, Mausherzgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

13015-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA HIP55 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIP55 fusion protein Ag4078
• Vollständiger Name: drebrin-like
• Berechnetes Molekulargewicht: 430 aa, 48 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC031687
• Gene symbol: HIP55
• Gene ID (NCBI): 28988
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIP55, also known as DBNL, ABP1, SH3P7, belongs to the ABP1 family. HIP55 plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. HIP55 acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes (PMID: 14729663). The N terminus of HIP55 contains a putative actin-binding domain found in drebrins, which are involved in brain development, and the C terminus contains an SH3 domain. Expect a band 55 kDa in size corresponding to HIP55 by western blotting.

Publikationen

Species	Application	Title
human,mouse	WB,IP	Acta Pharmacol Sin; The multifunctional adaptor protein HIP-55 couples Smad7 to accelerate TGF-β type I receptor degradation.; Authors - Yang Sun ; KD Validated
mouse	WB,IF,IP	J Neurosci; Drebrin-like (Dbnl) controls neuronal migration via regulating N-cadherin expression in the developing cerebral cortex.; Authors - Seika Inoue
		J Cell Sci; VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells.; Authors - Thomas Daubon
mouse	WB	J Microbiol; Effect of Salmonella treatment on an implanted tumor (CT26) in a mouse model.; Authors - Yun Misun M
human	IF	Nature; A cross-species proteomic map reveals neoteny of human synapse development; Authors - Li Wang