DBH Polyklonaler Antikörper

DBH Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10777-1-AP

Synonyme

Soluble dopamine beta-hydroxylase, EC:1.14.17.1, Dopamine beta-monooxygenase, Dopamine beta-hydroxylase, DBM

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Galerie der Validierungsdaten

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  WB analysis using 10777-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10777-1-AP (DBH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10777-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10777-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10777-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10777-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 10777-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 10777-1-AP

  Immunofluorescent analysis of HepG2 cells using 10777-1-AP (DBH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10777-1-AP

  Immunofluorescent analysis of HeLa cells using 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 10777-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DBH (10777-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

10777-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA DBH und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DBH fusion protein Ag1196
• Vollständiger Name: DBH
• Berechnetes Molekulargewicht: 64.8 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC017174
• Gene symbol: DBH
• Gene ID (NCBI): 1621
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DBH belongs to the copper type II ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of DBH to norepinephrine. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons(PMID:11857564).The DBH is glycosylated (63--67 kDa) while still in the membrane(PMID:2325165) and it can form a dimer(PMID:8546710).

Publikationen

Species	Application	Title
mouse	WB	Int J Mol Sci; Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism.; Authors - Shin Takasawa
human	WB	Peptides; Markers of the sympathetic, parasympathetic and sensory nervous system are altered in the human diabetic choroid.; Authors - Yuan-Jun Qin