DAO Monoklonaler Antikörper

DAO Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C12F4

Kat-Nr. : 66296-1-Ig

Synonyme

D amino acid oxidase, DAAO, DAMOX, DAO, OXDA

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Galerie der Validierungsdaten

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  WB analysis using 66296-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66296-1-Ig (DAO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of fetal human brain using 66296-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66296-1-Ig (DAO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 66296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66296-1-Ig (DAO Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human gliomas using 66296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66296-1-Ig (DAO Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of mouse cerebellum using 66296-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66296-1-Ig (DAO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 66296-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66296-1-Ig (DAO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, fetales humanes Hirngewebe, Hausschwein-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66296-1-Ig bindet in WB, IHC, ELISA DAO und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DAO fusion protein Ag6928
• Vollständiger Name: D-amino-acid oxidase
• Berechnetes Molekulargewicht: 347 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC029057
• Gene symbol: DAO
• Gene ID (NCBI): 1610
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

D-amino acid oxidase (DAO),with MW of 39 kDa, is a flavoprotein which uses flavin adenine dinucleotide as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. DAO may play a role in the pathophysiology of schizophrenia and act as a detoxifying agent which removes D-amino acids that accumulate during aging.DAAO exists in solution as a mixture of monomer-dimer(80 kDa) and even higher molecular aggregates in equilibrium(170 kDa), depending on the enzyme concentration and forms(PMID: 11130179/PMID: 9210639).