DAO Polyklonaler Antikörper

DAO Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 13273-1-AP

Synonyme

D amino acid oxidase, DAAO, DAMOX, DAO, OXDA

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Galerie der Validierungsdaten

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  WB analysis of A549 using 13273-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13273-1-AP (DAO Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13273-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13273-1-AP (DAO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 13273-1-AP

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 13273-1-AP (DAO antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 13273-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13273-1-AP (DAO Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 13273-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13273-1-AP (DAO Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, A549-Zellen, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13273-1-AP bindet in WB, IF, IHC, ELISA DAO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DAO fusion protein Ag3973
• Vollständiger Name: D-amino-acid oxidase
• Berechnetes Molekulargewicht: 347 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC029057
• Gene symbol: DAO
• Gene ID (NCBI): 1610
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

D-amino acid oxidase (DAO),with MW of 39 kDa, is a flavoprotein which uses flavin adenine dinucleotide as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. DAO may play a role in the pathophysiology of schizophrenia and act as a detoxifying agent which removes D-amino acids that accumulate during aging. DAAO exists in solution as a mixture of monomer-dimer(80 kDa) and even higher molecular aggregates in equilibrium(170 kDa), depending on the enzyme concentration and forms(PMID: 11130179/PMID: 9210639).

Publikationen

Species	Application	Title
mouse	WB	Brain Behav Immun; l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.; Authors - Danqing Xin
mouse	IF	Am J Physiol Cell Physiol; Functional role of histamine receptors in the renal cortical collecting duct cells.; Authors - Anastasia V Sudarikova
rat		Mol Cell Neurosci; d-Cysteine promotes dendritic development in primary cultured cerebellar Purkinje cells via hydrogen sulfide production.; Authors - Takahiro Seki
human,rat	WB,IHC	Am J Physiol Renal Physiol; Renal Histaminergic System and Acute Effects of Histamine Receptor 2 Blockade on Renal Damage in the Dahl Salt-Sensitive Rat; Authors - Denisha R Spires

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (03-14-2023)

It does not work in WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2, mouse liver, mouse kidney, mouse brain

DAO Antibody Western Blot validation (1:1000 dilution) in HepG2, mouse liver, mouse kidney, mouse brain (Cat no:13273-1-AP)