DAO Polyklonaler Antikörper

DAO Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13273-1-AP

Synonyme

D amino acid oxidase, DAAO, DAMOX, DAO, OXDA



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausnierengewebe, A549-Zellen, Ratten-Cerebellum-Gewebe
Erfolgreiche Detektion in IHChumanes Kolongewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13273-1-AP bindet in WB, IF, IHC, ELISA DAO und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DAO fusion protein Ag3973
Vollständiger Name D-amino-acid oxidase
Berechnetes Molekulargewicht 347 aa, 39 kDa
Beobachtetes Molekulargewicht 39 kDa
GenBank-ZugangsnummerBC029057
Gene symbol DAO
Gene ID (NCBI) 1610
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

D-amino acid oxidase (DAO),with MW of 39 kDa, is a flavoprotein which uses flavin adenine dinucleotide as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. DAO may play a role in the pathophysiology of schizophrenia and act as a detoxifying agent which removes D-amino acids that accumulate during aging. DAAO exists in solution as a mixture of monomer-dimer(80 kDa) and even higher molecular aggregates in equilibrium(170 kDa), depending on the enzyme concentration and forms(PMID: 11130179/PMID: 9210639).

Protokolle

Produktspezifische Protokolle
WB protocol for DAO antibody 13273-1-APProtokoll herunterladen
IHC protocol for DAO antibody 13273-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Brain Behav Immun

l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.

Authors - Danqing Xin
mouseIF

Am J Physiol Cell Physiol

Functional role of histamine receptors in the renal cortical collecting duct cells.

Authors - Anastasia V Sudarikova
rat

Mol Cell Neurosci

d-Cysteine promotes dendritic development in primary cultured cerebellar Purkinje cells via hydrogen sulfide production.

Authors - Takahiro Seki
human,ratWB,IHC

Am J Physiol Renal Physiol

Renal Histaminergic System and Acute Effects of Histamine Receptor 2 Blockade on Renal Damage in the Dahl Salt-Sensitive Rat

Authors - Denisha R Spires

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hua (Verified Customer) (03-14-2023)

It does not work in WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HepG2, mouse liver, mouse kidney, mouse brain
DAO Antibody Western Blot validation (1:1000 dilution) in HepG2, mouse liver, mouse kidney, mouse brain (Cat no:13273-1-AP)